WebOct 27, 2024 · Phenotypic analysis in our protocol was based on two cellular fluorescent markers (DAPI and myosin) decorating nuclei and myotubes respectively. Quantification of the decorated surface allowed us to define and analyze four phenotypic criteria (cellular density, myotube quantity, fusion index, myotube size, and morphology). WebMyoblast fusion is essential for formations of myofibers, the basic cellular and functional units of skeletal muscles. Recent genetic studies in mice identified two long-sought membrane proteins, Myomaker and Myomixer, which cooperatively drive myoblast fusion.
MyoScreen, a High-Throughput - SAGE Journals
WebThe fusion index is defined as the number of myosin heavy chain expressing myotubes with greater than 2 nuclei divided by the total number of nuclei. This seems like … books on the 1929 stock market crash
National Center for Biotechnology Information
WebMay 8, 2024 · The following list of measurements were obtained for each image: myotube width, myotube length, fusion index, number of myotubes, cell density, and the number of nuclei per myotube. Myotubes were classified as elongated structures containing three or more nuclei within a single membrane structure. WebMyoCount: a software tool for the automated quantification of myotube surface area and nuclear fusion index [version 1; peer review: 2 approved] David P. Murphy 1, Thomas Nicholson2, Simon W. Jones2, Mary F. O'Leary 3 1Department of Clinical and Movement Neurosciences, Institute of Neurology, University College London, London, UK WebJan 21, 2024 · We have developed MyoCount, an open-source program that runs via the freely available MATLAB Runtime and quantifies myotube surface area and NFI. MyoCount allows the user to adjust its parameters to account for differences in image quality, magnification and the colour channels used in generating the image. books on thanksgiving for kids