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Myotube fusion index analysis automated

WebOct 27, 2024 · Phenotypic analysis in our protocol was based on two cellular fluorescent markers (DAPI and myosin) decorating nuclei and myotubes respectively. Quantification of the decorated surface allowed us to define and analyze four phenotypic criteria (cellular density, myotube quantity, fusion index, myotube size, and morphology). WebMyoblast fusion is essential for formations of myofibers, the basic cellular and functional units of skeletal muscles. Recent genetic studies in mice identified two long-sought membrane proteins, Myomaker and Myomixer, which cooperatively drive myoblast fusion.

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WebThe fusion index is defined as the number of myosin heavy chain expressing myotubes with greater than 2 nuclei divided by the total number of nuclei. This seems like … books on the 1929 stock market crash https://dfineworld.com

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WebMay 8, 2024 · The following list of measurements were obtained for each image: myotube width, myotube length, fusion index, number of myotubes, cell density, and the number of nuclei per myotube. Myotubes were classified as elongated structures containing three or more nuclei within a single membrane structure. WebMyoCount: a software tool for the automated quantification of myotube surface area and nuclear fusion index [version 1; peer review: 2 approved] David P. Murphy 1, Thomas Nicholson2, Simon W. Jones2, Mary F. O'Leary 3 1Department of Clinical and Movement Neurosciences, Institute of Neurology, University College London, London, UK WebJan 21, 2024 · We have developed MyoCount, an open-source program that runs via the freely available MATLAB Runtime and quantifies myotube surface area and NFI. MyoCount allows the user to adjust its parameters to account for differences in image quality, magnification and the colour channels used in generating the image. books on thanksgiving for kids

Analysis and quantification of in vitro myoblast fusion using …

Category:Enhancing Myoblast Fusion and Myotube Diameter in Human 3D …

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Myotube fusion index analysis automated

Improving quantification of myotube width and nuclear

WebApr 30, 2014 · The cells were stained with Giemsa. (D) Fusion index at 72 h of differentiation. Fusion index was calculated as the percentage of total nuclei that resided in cells containing 3 or more nuclei. (E) Average number … WebDec 16, 2024 · MyoCount can count MYH-positive cells with less than three nuclei in them to generate fusion index automatically; however, this number is not fully accurate because of the aforementioned issues with resolving nuclear clumps, counting myoblasts as myotubes, and defining myotube borders.

Myotube fusion index analysis automated

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WebDec 1, 2024 · A novel software tool, JADE, is presented in this work. Its aim is to lead the path to a full standardization and automatization of V&V processes for neutron-gamma Monte Carlo transport for... WebThe use of the LADD Multiple Stain and automated ImageJ analysis provides a rapid, cost-effective method for analyzing and quantifying myoblast fusion. Y. BN ... myotube cytoplasm appears dark purple (Figure 1F; arrowheads), while ... Fusion index of differentiating cells (Day 5) cultured in the presence or absence of BB94 (10 µM). (E ...

WebDec 16, 2024 · ViaFuse can detect the borders of myotubes and identify nuclear clumps which have been limitations of previous muscle-centric imaging software. The ViaFuse macros require little computer power or space to run and user inputs to the ViaFuse macros are minimal, thereby automating the analysis process in a quick, easy, and accurate fashion. WebMyoCount: A software tool for the automated quantification of myotube surface area and nuclear fusion index [version 1; referees: 2 approved] David Murphy, Thomas Nicholson, Simon Jones, Mary O'Leary Inflammation and Ageing Immunology and Immunotherapy Research output: Contribution to journal › Article › peer-review 2 Citations (Scopus)

WebMar 16, 2024 · Myoblast fusion, which is essential for muscle development, regeneration, and repair, can be assessed in vitro via the calculation of a fusion index. Traditionally, this requires use of either immunocytochemistry or fluorescently-labeled cytoskeletal staining, followed by microscopy and laborious analysis. The expense and time-consuming nature ... WebJun 10, 2024 · The Myotube Analyzer can be used for the analysis of fixed differentiated myoblast cultures with nuclear and MyHC staining. The app can calculate the fusion index, an already existing parameter, but also provides multiple new parameters to comprehensively describe myogenic potential in its output.

WebJan 21, 2024 · It is often desirable to characterise the morphology of myogenic cultures. To achieve this, the surface area of myotubes is often quantified, along with the nuclear fusion index (NFI). Existing methods of such quantification are time-consuming and subject to error-prone human input.

WebAug 15, 2014 · To evaluate myogenic differentiation, a fusion index was calculated to indicate myotube fusion. For each condition, the total number of nuclei and the number of nuclei incorporated into the myotubes were counted using 10 images from randomly chosen microscope fields populated with DAPI- and MyHC-stained cells. harv\u0027s air online ground schoolWebNational Center for Biotechnology Information harv\u0027s air fleetWebSep 1, 2024 · Reproducibility and sensitivity of the fully automated MyoScreen platform are highlighted in assays that quantitatively measure myogenesis, hypertrophy and atrophy, AChR clusterization, and intracellular calcium release dynamics, as well as integrating contractility data. harv \u0026 marv whale watching