WebBuffer system: One universal buffer: Up to 20 buffers: Double/multiple restriction digestion: Unlimited—all 176 enzymes are 100% active in one buffer: ... * 0.5 mM ATP is required for T4 DNA Ligase activity. Complete digestion in 5–15 minutes. Double and multiple digestions in one buffer in 5–15 minutes: Saves time and effort, increasing ... WebLegal Disclaimer Notice This legal disclaimer applies to purchasers and users of Bourns® products manufactured by or on behalf of Bourns, Inc. and P[Z H ISPH[LZ JVSSLJ[P]LS` …
T4 DNA Ligase Enzymatics
Web12 rows · Sep 17, 2024 · Salt-T4 DNA Ligase is 100% active in T4 DNA Ligase Buffer supplemented with 100 mM NaCl, NEBuffer r3.1 and HiFi Taq DNA Ligase Buffer (when … WebSep 17, 2024 · Salt-T4 DNA Ligase is 100% active in T4 DNA Ligase Buffer supplemented with 100 mM NaCl, NEBuffer r3.1 and HiFi Taq DNA Ligase Buffer (when supplemented with ATP). Salt-T4 DNA Ligase has reduced activity in other NEB restriction enzyme and ligase buffers, however, activity in these buffers can be restored when supplemented … the yajes tribe
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WebUnit Definition One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32 P] in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X T4 Polynucleotide Kinase Reaction Buffer with 66 µM [γ-32 P] ATP (5 x 10 6 cpm/µmol) and 0.26 mM 5´-hydroxyl-terminated salmon sperm DNA … WebFor fill-in reactions only: T4 DNA Polymerase can be used in NEBuffers 1.1, 2.1, 3.1 and CutSmart ® Buffer as well as NEBuffers 1-4 and T4 DNA Ligase Reaction Buffer.; For blunting reactions requiring removal of overhangs: T4 DNA Polymerase can be used in NEBuffers 1.1, 2.1, and CutSmart Buffer as well as NEBuffers 1, 2, and 4 and T4 DNA … WebT4 DNA Ligase: Size Part No. (Weiss units) M180A 100 M180B 500 M179A (High Conc.) 500 Ligase Buffer, 10X (C126A, C126B): The Ligase 10X Buffer supplied with this enzyme has a composition of 300mM Tris-HCl (pH 7.8), 100mM MgCl 2, 100mM DTT and 10mM ATP. The performance of this buffer depends on the integrity of the ATP. safetynet solutions crewe