WebThese studies reveal that bromodomain-containing protein 4 (BRD4), a bromodomain and extraterminal (BET) family member and an important epigenetic reader, is critical for enhancer-mediated profibrotic gene expression in HSCs ( 4 – 6 ). WebDec 31, 2013 · The BET protein family includes four proteins, BRD2, BRD3, BRD4 and BRDT, that bind to select acetylated lysine residues on histones and other proteins via two tandem ∼110 amino acid bromodomains, and thereby recruit additional proteins that regulate transcription [28] ( Figure 2 ).
(A) Crystal structure of the first bromodomain of BRD4 in complex …
WebMar 1, 2024 · Double immunofluorescence was used to determine Brd4 colocalization with NeuN (a neuronal marker). Thus, free-floating sections were blocked in 5% normal donkey serum for 2 h prior to incubation in a mixture of rabbit anti-Brd4 polyclonal antibody (1:500, Bethyl Laboratories, A301-985A) and mouse anti-NeuN (1:1000, Chemicon, USA) for 24 h. WebApr 30, 2015 · The mammalian bromodomain and extraterminal motif proteins (BETs) have drawn widespread interest as pharmacologic targets for the treatment of various diseases, including hematologic malignancies and solid tumors. 1-4 Within the BET family, BRD2, BRD3, and BRD4 are ubiquitously expressed in mammalian tissues, whereas BRDT is … sunova koers
Rabbit anti-BRD4 Antibody - Fortis Life Sciences
WebBRD4. Cell type Cell type Class Adipocyte Cell type 402-91 NA NA. Attributes by original data submitter Sample source_name MLS 402-91 cell type liposarcoma cells chip antibody BRD4 (Bethyl, #A301-985A, lot4) Sequenced DNA Library library_strategy ChIP-Seq library_source GENOMIC library_selection ChIP WebNov 2, 2024 · Complex crystal structure of BRD4-BD1 with compound BDF-1253. a The modification from Nitroxoline to compound BDF-1253 increased potency against the BRD4-BD1. b The 2Fo-Fc electron density map of ... WebSep 5, 2024 · Anti-BRD4 (A301–985A) antibody for ChIP was obtained from Bethyl Laboratories. PE-conjugated human PD-L1 (MIH1 clone; 12-5983-42) antibody was from Thermo Fisher. sunova nz